Question

When would you use a confocal microscope as opposed to an electronor light microscope?

Answer 1

Answer:

Confocal Microscope is used for cell structures in the micrometer range while Electron microscope is used in the range of nanometers

Explanation:

The confocal microscope makes use of optical sectioning in order to achieve a thin section  with the sample still intact. It is used for cells or structures inthe micrometer range such as the lisosomes. The lisosomes in a cell can be tagged with a flourescence dye and they would be seen using the confocal microscope. They provide a high-resolution image with all areas in focus throughout the field of view

The Electron Microscope on the other hand requires real sections, it makes use of transmitted electrons (electrons which are passing through the sample) to create an image. It is used in a range of nanometers, with it, you can view the nanoparticles inside a lisosome.