Eutrophication results in the death of trout and other fish as a result of asphyxiation from the lack of oxygen. Eutrophication involves the enrichment of water by nutrients salts such as phosphates, nitrates among others, that causes structural changes of the aquatic ecosystem such as; increased production of algae and aquatic plants, depletion of fish species, deterioration of water quality and other effects that impact negatively the aquatic ecosystem.
Thermodynamics is the correct answer.
Answer: B. Melting temperatures of primer should be between 55-80 degree Celsius.
Explanation:
Bacause the melting temperature controls the binding of the primers to your template DNA. At melting temperature 50% of the primer molecules are bound to their corresponding target sequence. If the difference in melting temperature between the two primers is too high, it might be difficult to find experimental conditions where both primers can bind to their target.
The leading strand is the strand of nascent DNA which is being synthesized in the same direction as the growing replication fork. A polymerase "reads" the leading strand template and adds complementary nucleotides to the nascent leading strand on a continuous basis.
