Answer:
The correct answer is -
II: lyse cells of interest, precipitate DNA
V. Digest (cut) genomic DNA with restriction enzyme
I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!
IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.
III Introduce constructs via transformation into competent E. coli cells.
Explanation:
The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.
The genomic library construction involved some major steps that are:
- Extract and purify DNA - lysing and separating the desired DNA of interest.
- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes
- cut phage (chromosome) with restriction enzyme
- Insert the fragments of DNA into vectors
- introduce these recombinant a host bacterium by transformation, creating a DNA library.
Answer: C
Explanation: I left a potato out exposed to air before and it turned brown. I forgot what it’s called but it’s definitely C
Answer;
Genes
The code for heredity is carried on genes in each organism's DNA.
Explanation;
Genes are portions of the genome that codes for a protein or an RNA, Hereditary information that is contained in nucleotide sequence of DNA. The coded information is copied into RNA (through transcription process) and translated into chains of amino acids which eventually link together to make proteins. The hereditary information is passed from one generation to another.
Answer: The correct answer is capillaries
Explanation:
capillaries are tiny branched blood vessels that links arterioles to venules and allows exchange of nutrients and gases between the blood and its surrounding tissues
