1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
Learn more about subcultured at
brainly.com/question/26525141
#SPJ4
Dangerous, rocky, fast moving water is called cataract. C<span>ataracts are sections where the river tumbles over rocks, and can be very dangerous. A well known cataract can be found in the Nile River. </span>
Answer: The pundit is using a NATIONAL DEFENSE ARGUMENT to justify the trade restriction.
Explanation: National defense argument is when someone is using the security of the nation to defend his argument, to be very important as the security of the nation is very important.
This type of argument always tends to use the fallacy of red herring to convince his audience about his argument. The argument sounds to be important as it used red herring to relate it's point to the security of the nation during wartime.
The pundit politician has used the fallacy of red herring to relate it's reason of increment in the tariff of semiconductor importation, to the security of the nation during war times. Therefore this is a national security argument.
Answer:
The country labeled with the number 1 on the map above is Spain, and it has a population of 47 million people.
Explanation:
Spain is represented clearly in the European Map. This country has got a population of about 47 million people. The current population of Spain is 46,740,474 as of Saturday, September 14, 2019, based on the latest United Nations estimates.
The one downside to environmental protection laws is that there are only few people who listens and follows them as their are only few people who shows a deep care and passion for taking care of the environment and following the environmental protection laws.