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3 years ago

What is the Cas9 enxyme used for?

Biology

1 answer:

frez [133]3 years ago

7 0

Answer:

RNA

Explanation:

CRISPR system was discovered by a Japanese scientist. Naturally bacteria and many other prokaryotes have CRISPR system in their genome which they use as a self defense against a pathogen which is invading them. It consists of inter spaced, short palindromic nucleotide repeats which encode a guide RNA (gRNA). This guide RNA (gRNA) recognizes the target sequence on the pathogen. The gRNA is followed by Cas9 enzyme which along with the gRNA plays a very important role in breaking genome of the pathogen. Once the target gene is cut down the rest of the DNA can be rejoined by non homologous end joining.

But nowadays, scientists are using this system for genome editing and incorporating desired gene between the two ends which are cut down by CRISPR Cas9 system.

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Calculate the final concentration of BSA in the problems below using the formula

Rudiy27

Answer:

A. $C_2=1.5\frac{mg}{mL}$

B. $C_2=0.075\frac{mg}{mL}$

C. $C_2=0.01\frac{mg}{mL}$

D. $C_2=0.001\frac{mg}{mL}$

Explanation:

Hello.

In this case, we must compute the final concentration in all the cases so we solve for it in the given equation:

$C_2=\frac{C_1V_1}{V_2}$

Thus, we proceed as follows:

A. Here, the final diluted solution includes the 300 μL of the 5 mg/ml-BSA and the 700 μL of TBS.

$C_2=\frac{300\mu L*5\frac{mg}{mL} }{(300+700)\mu L}\\\\C_2=1.5\frac{mg}{mL}$

B. Here, the final diluted solution includes the 50 μL of the 1.5 mg/ml-BSA, the 450 μL of water and the 500 μL of TBS.

$C_2=\frac{50\mu L*1.5\frac{mg}{mL} }{(50+450+500)\mu L}\\\\C_2=0.075\frac{mg}{mL}$

C. Here, the final diluted solution includes the 10 μL of the 1 mg/ml-BSA and the 990 μL of TBS.

$C_2=\frac{10\mu L*1\frac{mg}{mL} }{(10+990)\mu L}\\\\C_2=0.01\frac{mg}{mL}$

D. Here, the final diluted solution includes the 10 μL of the 0.1 mg/ml-BSA and the 990 μL of TBS.

$C_2=\frac{10\mu L*0.1\frac{mg}{mL} }{(10+990)\mu L}\\\\C_2=0.001\frac{mg}{mL}$

Best regards.

7 0

3 years ago

Tony Loves to swim everyday what is Tony a noun verb adjective subject <br>

Anastasy [175]

Answer:

noun

Explanation:

Tony is a person, place, or thing

8 0

3 years ago

Read 2 more answers

At what stage of the cycle does the cell copy its DNA?

Murljashka [212]

Answer:

S-Phase

Explanation:

During the S-Phase, the cell replicates its DNA. Interphase is then followed by Mitosis. Mitosis is when the cell separates its two sets of DNA and divides it into two more cells.

5 0

3 years ago

Mrs. Jones who has dementia. She continues to ask for her husband, whom you know passed away several years ago. How would you as

wlad13 [49]

Answer:

You tell her that her husband is away and will be coming back later

Explanation:

If you tell her he's dead, she won't believe you so just go along with it.

3 0

2 years ago

Which puffy plant produces instantly blooming beans

Brrunno [24]

Puffapod (if this is for Harry Potter )

8 0

2 years ago