Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
Answer:
The correct answer is option D. Permissive.
Explanation:
The permissive effect or interaction of a hormone is in which hormone can not show or exert its complete effect without the presence of another hormone on a specific target site or cell.
The interaction of a hormone can occur in two more ways - synergies and antagonism where synergy occurs when two hormones perform the same action on a target cell and impact are amplified, and antagonist has the opposite role in the presence of another hormone.
Thus, the correct answer is - d. Permissive.
primary division of the sea, which includes the whole mass of water subdivided into neritic and oceanic zones; also pertaining to the open sea (open water)
Answer:
red,orange,yellow,green,blue,indigo,violet
7 colors
Answer: both parathyroid hormone and calcitrol.
Explanation:
In the body calcium homeostasis, which is generally defined as the maintenance of specific internal conditions, is mediated by the endocrine system through hormonal control; hormones are usually specialized signal molecules derived from lipids or amino acids. This regulates the calcium flux between the bloodstream and the bone; and the hormones responsible, change the ratio of osteoclast activity to osteoblast activity -osteoblasts build bone while osteoclasts breakdown or degrade bone.
Hormones responsible include calcitonin (from special parafollicular cells in the thyroid gland), cacitrol (activated vitamin D) and the parathyroid hormone (from the parathyroid glands). These affect the absorption of calcium from the gut, or kidney reabsorption; both parathyroid hormone and calcitrol act by causing the release of calcium ions into the bloodstream as the bone is degraded by osteoclasts.
