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3 years ago

Which of the following would be the last step in the process of DNA fingerprinting?

Biology

2 answers:

storchak [24]3 years ago

8 0

Answer:

(D). DNA fragments containing repeats are labeled with radioactive probes.

Explanation:

DNA fingerprinting can be defined as a process, which involves identification of DNA characteristics of an organism as unique in all organisms as fingerprints.

There are total five steps in DNA fingerprinting, which include isolation of DNA from the cells, digestion of DNA with restriction enzymes, separation of DNA fragments in agarose gel, transfer of DNA fragments to a blotting paper, and labeling of DNA fragments with radioactive probes of specific sequence to see the results.

Thus, the correct answer is option (D) as represents that last step of DNA fingerprinting.

timofeeve [1]3 years ago

6 0

D. Fingerprinting involves binding radioactive probes to label DNA that has been digested by an endonuclease and separated out

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3 years ago

Comparing DNA Replication and Transcription

ozzi

The missing information has been completed as:

DNA Tempelate complementary DNA mRNA

3' TTACG 5' 5' AATGC 3' 5'UUACG 3'

3' AAUGC 5' 5' TTACG 3' 5'UUACG 3'

3' GGCGG 5' 5' CCGCC3' 5' CCGCC3'

' 3'TGCATCG5' 5' ACGTAGC 3' 5' ACGUAGC 3'

3' AGACTC 5' 5' TCTGAG 3' 5' AGACTC 3'

3'GATAAGA 5' 5' CTATTCT 5' 5' CTAUUCA 3'

3' CTGGCTAC5' 5' GACCGATG 3' 3'CUGGCUAC 5'

Explanation:

Some of the sequence of DNA given is in 3' to 5' direction, mRNA sequence is also given.

The DNA is double stranded helix which replicates by semi conservative mode. The two strands unwind to act as a template for other strands. In transcription the 3' to 5' strand acts as a template because RNA synthesis takes place in 5' to 3' direction. mRNA is single strand. It has nucleotides in it from which 3 base pairs acts as a code for amino acid.

The following table shows DNA strand having complementary sequaence and mRNA synthesized.

DNA Tempelate complementary DNA mRNA

3' TTACG 5' 5' AATGC 3' 5'UUACG 3'

3' AAUGC 5' 5' TTACG 3' 5'UUACG 3'

3' GGCGG 5' 5' CCGCC3' 5' CCGCC3'

' 3'TGCATCG5' 5' ACGTAGC 3' 5' ACGUAGC 3'

3' AGACTC 5' 5' TCTGAG 3' 5' AGACTC 3'

3'GATAAGA 5' 5' CTATTCT 5' 5' CTAUUCA 3'

3' CTGGCTAC5' 5' GACCGATG 3' 3'CUGGCUAC 5'

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3 years ago