The chromosomes are in their condensed, highly coiled form, where they can easily fall without overlapping and staining will show a banding pattern.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Cell maintain homeostasis through diffusion in isotonic solution. Isotonic means that the concentrations are the same on either side of the membrane, maintaining homeostasis.
Answer:
The correct answer would be:
- Genotype ratio: 1 (PP) : 2 (Pp) : 1 (pp)
- Phenotypic ratio: 3 (polka-dot tails) : solid colored tail
The genotype of both parents is Pp (heterozygous).
Thus, both the parent would produce two types of gametes which are P and p.
The cross would result in the production of offspring with three types of genotype PP, Pp, and pp in 1:2:1.
Offspring with PP and Pp will have a polka-dotted tail as it is the dominant trait.
Thus, the phenotype ratio would be 3 (polka-dotted tail) : 1 (solid colored tail).
