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3 years ago

Why o we need a reverse compliment for pcr amplifying?

Biology

1 answer:

navik [9.2K]3 years ago

4 0

Solution:

Since, DNA occurs as a double strand where each A is paired with a T and vice versa, and each C is paired with a G and vice versa. The reverse complement of a DNA sequence is formed by reversing the letters, interchanging A and T and interchanging C and G. Thus the reverse complement of ACCTGAG is CTCAGGT.

And DNA has two strands: Forward and reverse. Miseq is the technique to sequence the DNA for various purposes such as mutation detection, important for disease diagnosis. Hence, one need to find the mutations in both strands rather than only the forward to strand, to prevent false positives and other artifacts.

This is the required answer.

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How Do I find offspring in Punnett square?

Serggg [28]

When you cross the parents on the outside of the Punnett square, the letters inside the boxes are the offspring

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3 years ago

Define the following terms. Drag and drop the words into the appropriate blanks to complete the sentences.

FrozenT [24]

Answer:

1. replication

2. gene

3. strand polarity

4. translation

5. complementary base pairing

6. chromosome

7. antiparallel

8. transcription

9. allele

Explanation:

1. Replication is the process by which DNA is duplicated or the process of making another copy of DNA.

2. Gene is the physical unit of heredity i.e a segment of DNA transferred from parents to offsprings. A gene also encodes a useful products such as protein.

3. A DNA strand is a polar molecule, which is attributed to the phosphate group at the 5'-end of DNA and the hydroxyl group at the 3'-end of DNA.

4. Translation is the process whereby the information in a mRNA molecule is used to synthesize a protein.

5. Complementary base pairing is a phenomenon whereby Adenine (A) binds to thymine (T), guanine (G) binds to cytosine (C).

6. A chromosome contains of single long molecule of DNA coiled together around histone proteins. The chromosome is associated with different types of proteins.

7. In the double-stranded DNA molecule, one strand runs from 5' to 3' and the other runs from 3' to 5'. This is called ANTIPARALLEL nature of DNA.

8. Transcription is the process by which a DNA molecule is used to synthesize a mRNA single strand.

9. Allele is the alternative or contrasting form of a gene. Each allele is contributed by each parent

5 0

4 years ago

I need help and please explain

Ierofanga [76]

Breaking chemical bonds is typically an endothermic process, which means energy must be added (it must pull in energy)

Breaking of chemical bonds is always endothermic meaning it wants energy, so it takes energy from its surroundings and sucks it in. Think of 'endo' as inside. The chemical bonds want energy inside of it so it can break.

Forming of chemical bonds is exothermic, meaning it releases energy into its surroundings. Think of 'exo' as outside, or exterior, it releases its energy outside of itself.

I hope that makes some kind of sense :)

7 0

3 years ago

What describes the complex carbohydrate cellulose

Verdich [7]

Answer:

Cellulose and chitin

Explanation:

i think is the answer, not really sure

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3 years ago

What are the functions if the cell membrane

SVETLANKA909090 [29]

The main function of the cell membrane is that regulates and monitors the movement of specific substances into and out of the cell, permitting only some substances to move across and not allowing others.

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3 years ago