Inflammatory response can be defined is a reaction of the body to injury or destruction of tissues. This reaction serves to dilute, destroy or simply wall off the agent causing the injury and is localized around the area of injury.Inflammatory response takes place in the following steps:1. Tissue damage caused by injury or bacterial infection is detected.2. Vasodilators and chemotactic factors such as histamine are released.3. As a result, there is increased capillary permeability and blood flow to the area.4. There is migration of phagocytes to the site of infection.5. Phagocytes and specific serum proteins attack and destroy bacteria.<span>6. Inflammation dies down once intruder has been destroyed.</span>
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
The heart is beneath the breastbone in the chest cavity of the frog
The process is called digestion
<span> d. Functional capacity
Functional capacity is a psychological mechanism of an individual to thrive in an environment by self-care, like being able to cook, feed themselves, take a bath, wear clothes, attend specific events such as gatherings and particular themes, and others. </span>
