Question

CQ9. If you want to use PCR technique to amplify markers located on six unlinked locations on the chromosomes, how many total unique primer sequences do you need

Answer 1

Answer / Explanation:

To properly understand these answer, we will first identify what a PCR is.

A PCR simply refers to Polymerase chain reaction. They are tools used in identifying or focusing on DNA for the purpose of cloning or duplicating it.

Now referring back to the question asked,

The answer is 12, you need 12 different primer to successfully use PCR technique to amplify markers located on six unlinked locations on the chromosome.

It should be noted that we need two (2) primers to to amplify a single (1) PCR product.

Now considering the fact that we have 6 different product, it is implied that we will be using 12 unique primers.

We utilize one (1) primer for each base of the sequence. The primers are complementary to the template DNA and confer the specificity of PCR for a target sequence.

Answer 2

Answer:

This question includes the following options:

A.1 - B.3 - C.6 - D.8 - E. 12

PCR which stands for "Polymerase Chain Reaction" is a molecular biology procedure to quickly multiply a small sample of DNA into millions or billions of DNA copies for studying purposes.

"Unlinked" signifies that the markers are present in 6 various chromosomes, but to amplify those markers we would need (12) unique primer sequences, due to it is required to have the reverse and forward primers for every marker; thereby the answer is (E.)