Question

A strain of E. coli is genetically engineered in which the lacZ and lacI genes are removed and replaced with a gene encoding a fluorescent protein. A copy of the operon in which the protein product fluoresces green is inserted into the chromosome, and a copy in which the protein product fluoresces red is inserted into a plasmid. Both copies carry a wild-type lacO gene, and the cells of the strain have a single copy of the lacI repressor gene. If both fluorescent proteins are expressed, the cells fluoresce yellow (because a combination of red and green fluorescence appears as yellow), and if neither of the fluorescent proteins is expressed, the cells show no fluorescence. If the lacI gene in the genetically engineered fluorescent strain is mutated to an inactive form (lacI-), the cells would fluoresce _____ in the presence of an inducer and _____ in the absence of an inducer.

Answer 1

Answer:

the cells would fluoresce no color in the presence of an inducer and yellow in the absence of an inducer.

Explanation:

As LacI is mutated it will not repressor it. But as inducer will be needed to induce the fluorescence stain.