Question

Compare and contrast p-dependent and p-independent termination of transcription in prokaryotes.

Answer 1

Compare and contrast two mechanisms for transcriptional termination in bacteria.

(rho)p-dependent termination: requires rut (rho utilization site), rho protein binds, moves towards 3' end, DNA encodes GC rich for stem loop, RNApoly pauses, rho protein catches up and separates RNA-DNA hybrid

(rho)p-independent termination: Uracil-rich sequence causes RNApoly to pause, stabilized by NusA near open complex RNA exit, UA bonds to weak to hold, DNA-RNA hybrid dissociates AKA intrinsic termination

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