Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut wit
h restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be toA) insert the fragments cut with X directly into the plasmid without cutting the plasmid.B) cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid.C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.E) cut the plasmid with enzyme X and then insert the gene into the plasmid.
The lithosphere - rocks (or geosphere) The Earth's solid surface is the lithosphere. It includes continental and oceanic crust as well as the various layers of the Earth's interior.
