Answer:
A. CRISPR sequences in bacteria resemble those from bacteriophage and plasmid DNA. (The CRISPR sequences are derived from previously invading bacteriophages and pladmids.)
B. For reverse genetic studies, CRISPR /CAS can be used to replace wild type alleles with mutant alleles which can knock out the function of target genes. (CAS 9 introduces double strand break (DSB) in the target sequence which is then repaired by non homologous end joining (NHEJ). NHEJ causes deletion, insertion or frame shift mutation leading to knock out of the target genes.)
C. CRISPR is a bacterial sequence in the bacterial chromosome. ( The sequences are derived from bacteriophages that had infected the bacteria defore.)
D. The guide RNA guides CAS to specific DNA sequences. ( The guide RNA directs CAS nuclease to the target sequences for editing.)
E. The CRISPR/CAS system was discovered in bacteria, and it's natural function is the equivalent of an immune system in bacteria for remembering viral infections and for attacking viral DNA in future infections. (The CRISPR/CAS system provides acquired immunity to bacteria.)
Explanation:
see answer
Prokaryotes don't have a cell nucleus. While eukaryote organisms have a nucleus. Prokaryotes organisms thus are confined into single-cell organisms
Answer:
aorta, capillaries
Explanation:
Blood pressure is highest as its leaves the heart through the aorta and gradually decreases as it enters smaller and smaller blood vessels (arteries, arterioles, and capillaries).
The disadvantage of wolf captivation is that it restricts the sample size which prevents to make a conclusion on the species.
Explanation:
To study the behavior of the wolves that are kept in captivation during their feeding times, food enrichment programmes are performed. The feeding sessions were proved to be quite useful in studying the stereotype behavior of the wolf.
However, this study is experimented on small samples and on restricted environment that doesn’t represent the actual environment of a zoo where wolves are allowed to behave as per their nature.
