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The answer would be skeletal markers and DNA analysis.
Skeletal markers are often used to identify the biological gender of skeletal remains. The most commonly used skeletal markers can be observed in the skull and in the pelvic bone.However, the accuracy of his method is limited to adults only the differences between male and female skeletal markers usually become visibly only after maturation.
DNA analysis is more widely uses nowadays because with the technology of today, laboratories can now detect and X and Y chromosome-specific sequences, which help them identify the biological gender.
Action potential is a mechanism of transfer of ions across the plasma membrane down their elctrochemical gradient. Action potential is regenerated by the influx of ions such as sodium in case of voltage gated sodium channels. The voltage gated channels can be either open or closed depending on the action potential. Depolarization of membrane leads to the opening of these channels that allow the sodium ions to enter the cell down their electrochemical gradient. The threshold of action potential is reached when the entry of sodium ions out reach the exit of potassium ions from the cell. This leads to the generation of action potential.
An thing or compound or anything containing carbon