Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
The awnser would be D the tilt on the earth on its axis
You can tell if asthma is related to exercise
(i) If a person has a coughing and shortness of breath. This feels a few minutes after an activity
(ii) You feel constricted. When on the exercise you feel like you can’t get enough air in your lungs.
(iii) A person feels fatigue after training instead of being energized.
(iv) If a person lives in a dry environment the breath in air that is divided and colder than the other parts of your body.
Answer:
water is constantly moving across the earth. The water is strong enough to break down soil in tiny pieces. What’s happening is a process called erosion. Erosion is: the process of eroding or being eroded by wind, water, or other natural agents.
"the problem of soil erosion".
Water also carries sand and moves it around, this obviously changes the shape of the earths surface!
Explanation:
