Question

Read the scenario.

Answer 1

Answer:

C. He forgot to add primers.

Explanation:

The major components of a PCR include:

• A sample DNA which is the DNA that needs to be amplified.
• A DNA polymerase, an enzyme that synthesizes new DNA strands from the sample DNA.
• A primer which represents the starting sequence for the new strand of DNA to be synthesized.
• Free nucleotides in form of Deoxynucleoside triphosphates (dNTPs)

The graduate student forgot to add primers which are essential as a starting point on which the synthesis of new DNA strand is based. DNA ligase is not needed for PCR, but during DNA replication.

The correct option is C.