A sample DNA which is the DNA that needs to be amplified.
A DNA polymerase, an enzyme that synthesizes new DNA strands from the sample DNA.
A primer which represents the starting sequence for the new strand of DNA to be synthesized.
Free nucleotides in form of Deoxynucleoside triphosphates (dNTPs)
<em>The graduate student forgot to add primers which are essential as a starting point on which the synthesis of new DNA strand is based. DNA ligase is not needed for PCR, but during DNA replication.</em>
<span> A cell typically expresses only a fraction of its genes and the different types of cells in multi cellular organisms rise bc of its different set of genes are ex-pressed </span> <span> </span>