Answer:
The atmosphere is the largest reservoir of the nitrogen as it is composed of 78% of Nitrogen. Although 78%, this is not used by the organisms directly as the nitrogen molecule exists in nature in the form of divalent joined via triple bonds.
These triple bonds require a great amount of energy to be broken and used. Only a few prokaryotic organisms called nitrogen-fixing bacteria have the capability to break these triple bonds as they contain enzymes-nitrogenase complex which converts the atmospheric nitrogen to usable forms like ammonia, nitrates and nitrites. About 92% of the atmospheric nitrogen is fixed through this way rest through thunderstorms and Haber's process.
Thus, nitrogen-fixing bacteria is the answer.
In fetal life, the lungs are not expanded, so most of the blood from the right ventricle is shunted from the pulmonary artery to the aorta through the patent ductus arteriosus. With a few breaths the lungs expand, and blood flows from right ventricle to pulmonary artery into the lungs. Withdrawal of circulating prostaglandin from the mother plus bradykinins released from the expanding lung of the infant cause the ductus to close over a few minutes to hours. The closed ductus remains as the ligamentum arteriosum.
