Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
Answer:
B. Farmers can grow the crop using fewer chemical pesticides than traditional
crops.
Explanation:
Easily distracted, cannot wait to take turns, excessive fidgeting, excessive climbing and running. Hope this helps, good luck!
I would go with the last one, allowed scientists to better identify the location of fossils and change prior scientific knowledge
Strong, naive, perfect balance of strong and naive.
