Answer: DNA Ligase
Explanation:
During DNA replication, both the parental DNA strands are separated and act as templates for the synthesis of new strands. The formation of the new strands occurs in only 5'-3' direction and thus both the strands are synthesized in a different manner. One new strand with the template of 3'-5' direction will be synthesized continuously and is called a leading strand. The other strand with the template of 5'-3' direction will be synthesized discontinuously in the form of DNA fragments. These fragments are called Okazaki fragments and the strand is called a lagging strand. DNA ligase connects these fragments later to form a continuous lagging strand.
Therefore, DNA ligase is required for ligation of Okazaki fragments, and in absence of DNA ligase, the ligation of fragments will not occur and the newly synthesized strand will be discontinuous. However, if DNA ligase is not present in the reaction mixture, then the chances are that not all the newly synthesized DNA strands will be discontinuous. Only the DNA molecule with the lagging strand will be in the form of fragments.
Answer:
Length of the cells = diameter of field of view/ number of cells across the field of view
Explanation:
therefore 2.25= 4.5*X
X= 2.25/4.5
=0.5 number of cells
Gel electrophoresis is a method of genetic analysis which involves seperating segments of DNA based on their size and charge.
It involves cutting DNA with various enzymes, then placing the DNA in an agarose gel. An electrical current is then run through the gel. Because DNA is a polar molecule the DNA segments will travel through the agarose cell as the current is applied. Larger fragments will travel shorter distances than shorter fragments. Two plates with 2 different DNA samples will be conducted and the orientation of the DNA strands will be used to make an analysis by comparing the 2 plates.
Spread of disease is density dependent because it is more severe the greater the population density as the disease would spread more easily. If there is low population density it would spread slower.
