The formation of a phosphodiester bond between adjacent nucleotides is catalyzed by the DNA polymerases. They do this efficiently only if the incoming base on the incoming nucleoside triphosphate is complementary to the base on the template strand and can bind efficiently.
Explanation: DNA polymerase catalyze the formation of phosphodiester bonds between adjacent nucleotides in the DNA polymer being formed during DNA replication. The DNA polymerase catalyze the formation of poly nucleotide chains through the addition of new nucleotides from incoming deoxyneucleoside triphosphate. The polymerase reaction needs an appropriate DNA template to take place.
Each incoming neucleoside triphosphate first forms a base pair with a base in the template. Next, the DNA polymerase links the incoming base with the predecessor in the chain. Therefore, DNA polymerases are template directed enzymes.
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