Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Explanation:
As oxygen is consumed to provide energy, germinating seeds release carbon dioxide. ... HYPOTHESES: The experimental hypothesis is that germinating seeds will show a greater rate of respiration than control glass beads. Additionally, that at higher temperatures, the rate of cellular respiration in the seeds will increase.
Answer:
Explanation:
Law of conservation of mass:
According to the law of conservation mass, mass can neither be created nor destroyed in a chemical equation.
Explanation:
This law was given by french chemist Antoine Lavoisier in 1789. According to this law mass of reactant and mass of product must be equal, because masses are not created or destroyed in a chemical reaction.
For example:
When food is eaten it is digested by converting it into smaller particles. These smaller particles are than digest and gives the energy to our body. The energy which produced is just come from our food on this base we can say that mass neither created nor destroyed but it change one form to another, just like the form from food to energy.
Another example:
In given photosynthesis reaction:
6CO₂ + 6H₂O + energy → C₆H₁₂O₆ + 6O₂
there are six carbon atoms, eighteen oxygen atoms and twelve hydrogen atoms on the both side of equation so this reaction followed the law of conservation of mass.
Answer:
I think its b but I'm not sure
don't hate me if I'm wrong
