Answer:
The single guide RNA forms hydrogen bonds with DNA, while Cas9 hydrolyzes phosphodiester bonds
Explanation:
The base pairing between nucleic acid strands (either DNA or RNA) is through hydrogen bonds between nucleotide bases. In DNA, Adenine always forms two hydrogen bonds with Thymine, while Guanine always forms three hydrogen bonds only with Cytosine. Moreover, adjacent nucleotides in the same strand are covalently linked by phosphodiester bonds (i.e., covalent bonds between the 5' phosphate group of one nucleotide and the 3'-OH group of another). The CRISPR/Cas9 genome editing systems make use of single-guide RNAs (sgRNAs) that interact with DNA through hydrogen bonds. These sgRNAs have perfect complementarity to the target DNAs in order to bind them. On the other hand, Cas9 is an enzyme that hydrolyzes phosphodiester bonds in both DNA strands very precisely and accurately by using a sgRNA complementary to a specific DNA sequence.
Answer:
During the process of glycolysis in cellular respiration, glucose is oxidized to carbon dioxide and water. Energy released during the reaction is captured by the energy-carrying molecule ATP (adenosine triphosphate).
Two weeks before her menstrual period, margeret often suffers from PMS. The abbreviation PMS stands for Premenstrual syndrome .
Premenstrual syndrome is a combination of emotional, physical, psychological, and mood disturbances that occur after a woman's ovulation, typically ending with the onset of her menstrual flow. The most common mood related symptoms are irritability, depression, crying, oversensitivity, and mood swings.
Answer:
C
Explanation:
A, B, and D are done by other things, but acid can also be used to get rid of unneeded/wanted bacteria or organisms.
Answer:
is the car's acceleration.
Explanation:
Initial velocity of the driver = u = 14 m/s
Final velocity of the driver = v = 5.5 m/s
Duration of time in which 14 m/s velocity is changed to 5.5 m/s = t = 6.0 s
Acceleration of the driver = a
Using first equation of motion: v = u + at




Negative sign indicates the that is deceleration is occurring that is speed of the car is decreasing.