Answer:
I would have to say recharge of the aquifer by using ground water after some precipitation.
Explanation:
Melting snow or rain increases ground water that percolates down to replenish the aquifer.
Elevation changes causes the aquifer to flow up in low spots/springs on the surface. And the cycle repeats through evaporation and plant respiration.
<h3><u>Answer;</u></h3>
Gene therapy to correct defective genes that cause diseases.
<h3><u>Explanation</u>;</h3>
- Gene therapy refers to the procedure that involves the introduction of nucleic acids (DNA or RNA) into the cells of an organism for the purpose of correcting abnormalities, such as a mutations or in other words to treat a genetic disease.
- Gene therapy entails bringing a normal and functional gene known as a trans-gene into a cell with altered gene. Another method can bring RNA capable of partially regulating or blocking the expression of an altered gene.
- The nucleic acids are introduced into the patient's cells by means of a viral vector or injected directly into the cells in the form of naked DNA.
This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
The answer is carbohydrate.
Gene expression is about making mRNA which is then translated into a protein. If the mRNA that was expressed is mis-read it may or may not have an affect on gene expression. It will have a negative effect if:
1. The protein produced is required for gene expression (e.g. the DNA polymerase)
2. The protein produced is another critical protein that the cell cannot live without.
3. If the question is more about constantly misreading during translation, eventually the cell would die because the wrong proteins would be made (or not made at all).
