Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer: Mouth.
Explanation:
The mouth is known to be performing both the tasks of chemical digestion and mechanical digestion.
The food is ingested through mouth and the teeth perform the process of mechanical digestion by breaking down the food particles into smaller particles.
Then the saliva gets mixed into the food and the enzymes(amylase) present in the saliva helps in the digestion of carbohydrates.
So, mouth is the organ that performs the process of both mechanical and chemical digestion.
An open fracture is most accurately defined as a fracture in which the overlying skin is no longer intact. A fracture is normally the break in the continuity of the bone. It is also called a compound fracture, in which the fracture is an open wound or break in the skin near the site of the broken bone. Immediate treatment for an open fracture focuses on preventing infection at the site of the injury.
An example of the liver is an organ.
The liver is positioned on the right side of our stomach, and is colored reddish-brown. The liver aid us to get rid of damaging substances and benefits us to keep the body natural of contamination. The liver also is treated as a gland, due to generating bile (needed to consume fats). The liver can likewise help us wipe out aged blood cells, eliminates unhealthy substances such as alcohol and narcotic types, and also serves in balancing iron and vitamins in us.
Alright cool... and saw what..?
