Sympathetic nervous system under the Autonomic nervous system (ANS) is responsible for the physiological changes you experience when startled by a loud noise.
Autonomic nervous system (ANS) is part of the peripheral nervous system (PNS) and is responsible for control of vital functions.
ANS possesses three divisions, they are : Sympathetic nervous system (SYNS), parasympathetic nervous system and enteric plexus. Sympathetic nervous system is responsible for flight and fight responses.
SYNS also performs such tasks as relaxing the bladder, speeding up heart rate, and dilating eye pupils.
The ganglions associated with SYNS mostly arise from the thoracic and lumbar regions of the spinal cord.
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Answer:
You can use the quantitative Reverse Transcription Polymerase Chain Reaction PCR (RT-qPCR) method in order to analyze the Oct4 mRNA level.
Explanation:
RT-PCR is the gold standard technique to analyze the expression levels of one particular messenger RNA (mRNA) molecule. In this technique, the total RNA is firstly extracted and transcribed into complementary DNA (cDNA). Subsequently, the gene sequence of interest is then amplified in a standard PCR technique that uses as control one or more genes in order to measure the relative expression levels of the target mRNA, in this case, for example, the Oct4 mRNA.
