The second edition of bergey's manual of systemic bacteriology is largely based on of prokaryotic classification.
<h3>What does bergey's manual of systemic bacteriology talk about?</h3>
Since publication of the first edition of Bergey's Manual of Systematic Bacteriology, it has become recognized throughout the world as the principal monographic work in the field of prokaryotic biology. Like a dictionary to a writer, the Manual is usually the first reference that a microbiologist consults when questions arise regarding the characteristics of an unfamiliar species or an unknown strain that bears some similarity to a more familiar one.
While the first edition has served the community well for many years, it has become outdated. As a result, the editorial board of Bergey's Manual Trust, in collaboration with more than 500 of the world's leading authorities in prokaryotic systematics, is in the process of revising Bergey's Manual of Systematic Bacteriology so that it reflects current thinking and advances in the field.
With this information, we can conclude that Phylogenetic classification is now broadly accepted as the preferred method of representing taxonomic relationships among prokaryotes and eukaryotes alike.
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I will tell them I will try my best and it may take some time
Answer:
You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?
Personal Protective equipment needed; No special precautions are necessary when working with methicillin-resistant staphylococcus aureus, staphylococcus aureus is a bio-safety level
Three different culturing media; to include;
- Etest Method
- Disc Diffusion
- Latex Agglutination.
Properly enriched selective media is used.
The most accurate method for identifying Merthicillin-resistant staphylococcus is to test for the presence of mecA gene by PCR.
The best specific media is latex agglutination test and oxacillin salt agar screen.
How to determine if the culture was contaminated;
It can be spotted by the turbidity of the growth medium or the floating branching mycelia, bacteria contamination can be detected under 10× microscope within few days of contamination.
Step to take if contamination is detected;
Biological contamination is a serious issue and being able to correctly identify different types of contamination is an essential first step in ensuring the safety of your culture.
Explanation:
The literature on methicillin susceptible testing is extensive, and often conflicting in recommendations regarding the most important method for routine use. this is partly because the various studies of phenotypic methods have included different strains, which may differ significantly in heterogeneity and behave differently under particular test condition.
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