Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
G-T-A-G-C-T
Explanation:
A always pair with T and G always pairs with C.
Carbon fixation is the process in which plants remove the carbon from atmospheric carbon dioxide and turn it into organic molecules like carbohydrates.
Because different type of plants are located in regions with different conditions they are different types of carbon fixation. Plants that live in arid regions need to conserve water, while plants that live in more moist conditions will not need to conserve water.
The carbon fixation in C3 plants is one-step process. An oxidation reaction occurs because of the enzyme RuBisCo. During the oxidation some of the energy used in photosynthesis is lost in a process known as photorespiration.
Hi!
The answer is B) it should be repeated by other scientists.
Hope this helps!
-Payshence xoxo
Being born with the genes, and evolving to reach food easier
Maybe, IDK Tbh XD
