Question

When Avery and his colleagues had obtained the transforming factor from the IIIS virulent cells, they treated the fraction with proteases, ribonuclease, and deoxyribonuclease, followed by the assay for retention or loss of transforming ability.
What was the purpose of these experiments?

Answer 1

Answer:

to find out the exact molecular species of the transforming principle

Explanation:

As demonstrated by Avery and his colleagues, their aim and purpose is to find out an determine the exact molecular species of the transforming principle.

In the quest to find out, they obtained IIIS virulent cells and treated the fraction with proteases, ribonuclease and deoxyribonulease.

The purpose of these treatment was to hydrolyze peptide bonds of proteins, cleave RNA and catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone of the virus cell, thus degrading the DNA in the virus cell respectively.

This process was followed by assay for retention or loss of transforming ability. We can see that it is glaring that the purpose of these experiment was to find out the exact molecular species of the transforming principle.