<h2>Answer:</h2>
The water will move in and out freely. The material transport which is due to the water transport will be affected.
<h3>
Explanation:</h3>
- Water is a partially polar molecules. Many ionic compounds and ions travel inside and outside the cell due to attachment with water molecule.
- As well as water movement is controlled due to its polar nature through cell membrane. and the cytoplasm and outer environment is separate due to non polar cell membrane.
- If the water become non polar then the movement through cell membrane will be easy for water. And the molecules dissolved in water will also be transported uncontrollably.
Answer:
❥ Ripe pawpaws give off a wonderful fruity aroma. If the pawpaw does change color, it will likely turn a lighter shade of green, possibly with some yellowing. A more reliable indicator is feeling. The fruit will begin to feel softer, much as a peach or avocado.
❥ Your ripe tomato will give slightly to the touch. It shouldn't be soft but rather a little tender. Because tomatoes ripen from the inside out, this is a good indicator that it's ready.
Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Because since developing countries are still growing, when they finally get to the level of some more advanced countries the country as a whole feels a "greater growth"
Science locates necessary info about how the universe works. Engineering invents practical devices and systems using the knowledge learned by science. Technology is the collection of <span>products of engineering that can be used to solve problems and perform useful </span><span>tasks.</span>
