Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Buddha is shown symbolically through a wheel or footprints as well as Mahayan symbols. The Eight-Spoked Dharma Wheel portrays, In which the Buddha is turning the Wheel of Truth. It is the most important of all the buddhism symbols. Footprints represents the physical presence of Buddha.
Answer:
7 followed by 27 zeros
Explanation:
The body of an adult man weighing 70 kg is made up of approximately 6.7 • 10 ^ 27 atoms.
The body of an adult male contains approximately 57% water, but if we look by weight, hydrogen is only 11%, while if we look at the mutual ratio of atoms in water, there are a total of 67% hydrogen atoms.
In this way, most of the weight (mass) of the human body comes from oxygen, but most of the atoms in the body are hydrogen.
Microvilli which line down the luminal surface of those epithelial cells represent the resorptive function of the proximal tubule.
<span>The high quantity of mitochondria inside the epithelial cells of the proximal convoluted tubule is needed in order to supply the energy for the active transport of sodium ions (to enter the cell from the luminal side).</span>
Answer:
C. 200 billion
Explanation:
The milky way galaxy is estimated that there is 200 billion stars
