Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
B. it makes your results more convincing
Explanation:
It is important to conduct multiple trials in an experiment because it makes results more convincing.
Multiple trials removes and spreads error from one or more steps in experiment.
- The ability to reproduce result in an experiment is known as precision.
- Precision is very important in experiments.
- When a scientist carries out multiple trails in an experiment, it helps to remove any likely error that might have been carried into the experiment.
- Multiple trials are key to efficiency of experiments.
- From the results we can confidently state findings based on statistically backed data obtained.
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Population because you are holding it
Group of people could have a very specific challenge having to do with the person or the task - need to watch the same person to see how they change; Ex: some type of physical or cognitive challenge and want to see their progression
Hope this helps
Insect immunity doesn't include the property of an enzyme activation of pathogen-killing chemicals.
What is enzyme activation of pathogen?
Due to various trehalose's importance in pathogenic organisms, there has lately been a surge of interest in trehalose metabolism for therapeutic applications.
- TPP (trehalose-6-phosphate phosphatase) is an important enzyme in the most important biosynthetic pathway (OtsAB).
- The enzyme properties of recombinant TPPs from five key nematode and bacterial pathogens, including three new members of this protein family, are compared here.
- The kinetics of trehalose-6-phosphate hydrolysis demonstrate that all five enzymes exhibit burst-like kinetic behaviour, defined by a drop in enzymatic rate beyond the pre-steady state.
- Multiple global conformational changes in members of this enzyme family during substrate processing can explain the observed super-stoichiometric burst amplitudes.
Hence, the correct answer is option A
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