I believe that the answer is D. If something has evolved, it can not interbreed with its old species. I think this is right!
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Answer: salt
Freshwater is defined as having a low salt concentration, usually less than 1%.
Explanation:
Freshwater refers to the bodies of water that contain very low levels of dissolved salts. So, freshwater are unique for their low salt content (about 0.5% of salt) compared to the sea water with high salt content.
Examples of freshwater include rivers and spring