Nucleotides are added to the growing strand one at a time in the precise sequence dictated by the existing template strand. adenine and thymine are always paired with each other in the Watson-Crick DNA model, and cytosine is always paired with guanine.

The nucleotide sequence of DNA serves as a template for the nucleus, where mRNA is synthesised. The RNA polymerase II enzyme is responsible for this reaction's catalysis and needs nucleotide triphosphates as its substrates. Transcription is what happens in the nucleus when DNA is converted into mRNA. The cytoplasmic synthesis of proteins is controlled by the mRNA. The cytoplasm is where the mRNA that is produced in the nucleus attaches to the ribosomes after being transported from the nucleus. The nucleotide sequence of the mRNA serves as a guide for the assembly of proteins on ribosomes. The cytoplasm receives a "message" from the nucleus via mRNA. The nucleotide sequence of the mRNA, which is complementary to the nucleotide sequence of the messenger RNA, encodes the message.

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