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NemiM [27]
3 years ago
7

How did scientists discover that all living things are made of cells?

Biology
1 answer:
Arisa [49]3 years ago
4 0
They studied apes they saw frogs doing that nutrients are needed for the plants living is when you can breathe and nonliving is things that dont talk or walk
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One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on
andreyandreev [35.5K]

Answer:

Reduced in holding time of decolrization step and also used less Alcohol because decolrization step is important in Gram's staining.The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative..

Explanation:

Gram' staining is a technique used in microbiology labs to differentiate between Gram's positive and negative

<u>Gram-positive bacteria :</u>Stain dark purple due to retaining the primary dye called CV in the cell wall.

<u>:Gram-negative bacteria </u>Stain red or pink due to retaining the counter staining dye called Safranin or neutral red.

There are four basic step in Gram" staining

1) Application of the Primary Stain to a Heat-Fixed Smear of Bacterial Culture

2)Addition of Gram's Iodine

3)<u>Decolorization with 95% Ethyl Alcohol:</u>Alcohol or acetone dissolves the lipid outer membrane of Gram-negative bacteria, thus leaving the peptidoglycan layer exposed and increases the porosity of the cell wall. The CV-I complex is then washed away from the thin peptidoglycan layer, leaving Gram-negative bacteria colorless.

On the other hand, alcohol has a dehydrating effect on the cell walls of Gram-positive bacteria that causes the pores of the cell wall to shrink. The CV-I complex gets tightly bound into the multi-layered, highly cross-linked Gram-positive cell wall thus staining the cells purple.

The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative. Under-decolorization occurs when the alcohol is not left on long enough to wash out the CV-I complex from the Gram-negative cells, resulting in Gram-negative bacteria to appear Gram-positive.

7 0
3 years ago
How can i use point mutation in a sentence
Flura [38]
It was at that POINT MUTATION began
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3 years ago
In our lives, we rarely experience temperatures that are above 373 K (100 °C) or below 273 K (0 °C). Investigate how much diffus
Mariulka [41]

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Global and I have been working for the last three years and have been working with a company based based on the following areas for big growth in the UK with the team in North

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7am and the family will have a better understanding than the other in the UK or elsewhere in the UK or Europe and are not liable for any of the services or services that are in the UK next week and will also not have to pay for the distance and the family will also need a lot of information to help with the team to work with the team to work with the team to work with the team and provide a service for them in the workplace to the workplace as the service is not fully functioning properly and they will not be allowed to be charged for the service charge to the council tax and the council tax will not be paid for the council tax which is not covered by the council tax which is to be paid by the council tax and the council tax has been paid for the property to be paid by the council tax and the council tax has been paid for the property to be paid by the council tax and the council tax has been paid for the property to be paid by the council tax and the council tax has been paid for the property to be paid

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3 years ago
Pineapple monocot or dicot
Ira Lisetskai [31]

the answer is pineapple

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3 years ago
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Which is the best description of biology? A. The study of humans and animals B. The study of all living things C. The study of p
Reika [66]

Answer:

<h3>B. The study of all living things.</h3>

Explanation:

Biology is the SCIENTIFIC study of life. It is a natural Science with a broad scope such as anatomy, physiology , morphology e.t.c.

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