All categories

2 years ago

What is RFLP? What are some of the limitations of this technique?

1 answer:

3 0

In molecular biology, restriction fragment length polymorphism, orRFLP, is a technique that exploits variations in homologous DNA sequences. It refers to a differencebetween samples of homologous DNAmolecules from differing locations ofrestriction enzyme sites, and to a related laboratory technique by which these segments can be illustrated. InRFLP analysis, the DNA sample is broken into pieces and (digested) byrestriction enzymes and the resultingrestriction fragments are separated according to their lengths by gel electrophoresis. Although now largely obsolete due to the rise of inexpensive DNA sequencing technologies, RFLP analysis was the first DNA profilingtechnique inexpensive enough to see widespread application. RFLP analysis was an important tool in genome mapping, localization of genes forgenetic disorders, determination of riskfor disease, and paternity testing.
Hope it helps..

You might be interested in

In humans, Rh-positive individuals have the Rh antigen on their red blood cells, while Rh-negative individuals do not. If the Rh

bixtya [17]

Answer: 60%

Explanation:

In population genetics, the Hardy-Weinberg Principle states that the genetic composition of a population remains in equilibrium as long as no natural selection or other factors are active and no mutations occur.

The frequencies of the genotypes of an individual locus will be set to a particular equilibrium value. It also specifies that these equilibrium frequencies can be represented as a simple function of the allelic frequencies at that locus. In the simplest case, with a locus with two alleles A and a, with allele frequencies of p and q respectively, the principle predicts that the genotypic frequency for the dominant homozygous AA is p^2, that of the heterozygous Aa is 2pq and that of the recessive homozygous aa, is q^2. The allele frequency of a is represented as p and the frequency of recessive allele is q.

The three genotypes AA : Aa : aa appear in a ratio p² : 2pq : q². If we add them up, we get the unit:

p^2 + 2pq + q^2 = (p + q)^2 = 1

And p + q = 1

Rh-positive genotypes are represented as AA (homozygous dominant) or Aa (heterozygous) since the presence of a single dominant allele is sufficient to express the phenotype. While Rh-negative genotypes are represented as aa.

If 84% of the population is Rh-positive, that means that this percentage includes all those who are AA and Aa. Then 16% are aa.

F(aa)=q^2=0.16

then F(a)=q=0.4

And since p + q = 1, p + 0.4 = 1, then p is 0.6

We can also calculate the rest, then F(AA)=p^2= 0.36

So F(Aa)= 2pq = 2 x 0.6 x 0.4 = 0.48

Notice that 0.36 + 0.16 + 0.48 = 1

3 0

2 years ago

Which subatomic particle is positively charged?

EastWind [94]

Answer:

Proton

Explanation:

Neutron are neutral, ion I am not sure but are not positive, and electron are -.

3 0

3 years ago

Read 2 more answers

Which level of organization is characterized by a group of cells that work together to perform a common function

Whitepunk [10]

Hey there! :D

Organization in organisms is like this:

cells => tissues => organs => organ systems => organisms

Since we are talking about a group of cells, this would be a tissue. Don't let the word 'function' confuse you. Tissues do functions as well as an entire organ or organ system.

It is helpful to use the flow chart when asked questions like these, so make sure to memorize it! It will be helpful in any level of science.

I hope this helps!

~kaikers

4 0

2 years ago

What is the purpose of degerming? Does it completely eliminate microbes?

madam [21]

Answer:

Explanation:

The degerming is the act of handwashing. In this process, the microbes are removed by scrubbing the hands with a disinfectant usually a chemical, sterilizer, soap or detergent. This process will help in removing the microbes from the skin. It is a mechanical process of removing the germs or microbes without killing them.

This process provides a temporary protection from microbes.

3 0

3 years ago

Areolar connective tissue is often considered to be the prototype of connective tissue proper

Kryger [21]

The statement above is TRUE.
Connective tissues are the most abundant primary tissues in the human body. There are four types of connective tissues, they are fat & fibrous, cartilage, blood and bone. Areolar is the most common connective type and is considered as the prototype of connective tissue proper.

8 0

3 years ago