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Anon25 [30]
3 years ago
8

What is RFLP? What are some of the limitations of this technique?

Biology
1 answer:
bearhunter [10]3 years ago
3 0
In molecular biology, restriction fragment length polymorphism, orRFLP, is a technique that exploits variations in homologous DNA sequences. It refers to a differencebetween samples of homologous DNAmolecules from differing locations ofrestriction enzyme sites, and to a related laboratory technique by which these segments can be illustrated. InRFLP analysis, the DNA sample is broken into pieces and (digested) byrestriction enzymes and the resultingrestriction fragments are separated according to their lengths by gel electrophoresis. Although now largely obsolete due to the rise of inexpensive DNA sequencing technologies, RFLP analysis was the first DNA profilingtechnique inexpensive enough to see widespread application. RFLP analysis was an important tool in genome mapping, localization of genes forgenetic disorders, determination of riskfor disease, and paternity testing.
Hope it helps..
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The cells that develop to form the outer lining of an animal's body make up the _[blank]_.
Radda [10]

Answer:

The answer is ectoderm. The mesoderm is the middle layer of lining. The endoderm is the innermost layer. You can remember it by:

Endo/ sounds like inner

Meso/ M for middle

Ecto/ sounds like exo, bugs have exoskeletons that are on the outside.

Explanation:

4 0
3 years ago
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Murrr4er [49]
The control group is the part of an experiment that does not receive the variable and is used for comparison.
3 0
3 years ago
Starting with a protein that has been inserted into the Endoplasmic reticulum (ER) membrane with the Amino (N) terminal in the E
Vika [28.1K]

Answer and Explanation:

Ribosomes are the primary structure for protein synthesis. They can be found in the rough endoplasmic reticulum or floating in the cytosol.  

Free ribosomes are not attached to any cytoplasmic structure or organelle. They synthesize proteins only for internal cell use. Other ribosomes are attached to the membrane of the endoplasmic reticulum and they are in charge of synthesizing membrane proteins or exportation proteins. Free and attached ribosomes are identical and they can alternate their location. This means that although free ribosomes are floating in the cytosol, eventually, they can get attached to the endoplasmic reticulum membrane.  

Synthesis of proteins that are destined to membrane or exportation starts in the cytoplasm with the production of a molecule portion known as a <u>signal aminoacidic sequence</u>. This signal sequence varies between 13 and 36 amino acids, is located in the <u>amino extreme</u> of the synthesizing protein, and when it reaches a certain length, it meets the <u>signal recognizing particle</u>. This particle joins the signal sequence of the protein and leads the synthesizing protein and associated ribosome to a specific region in the Rough endoplasmic reticulum where it continues the protein building. When they reach the membrane of the endoplasmic reticulum, the signal recognizing particle links to a receptor associated with a pore. Meanwhile, the ribosome keeps synthesizing the protein, and the enlarged polypeptidic chain goes forward the reticulum lumen through the pore. While this is happening, another enzyme cuts the signal sequence, an action that requires energy from the ATP hydrolysis. When the new protein synthesis is complete, the polypeptide is released into the reticulum lumen. Here it also happens the protein folding (which is possible by the formation of disulfide bridges of proteins are formed) and the initial stages of glycosylation (the oligosaccharide addition).  

Once membrane proteins are folded in the interior of the endoplasmic reticulum, they are packaged into vesicles and sent to the Golgi complex, where it occurs the final association of carbohydrates with proteins. The Golgi complex sends proteins to their different destinies. Proteins destined to a certain place are packaged all together in the same vesicle and sent to the target organelle. In the case of membrane proteins, they are packaged in vesicles and sent to the cell membrane where they get incrusted.  

There are certain signal sequences in the <u>carboxy-terminal extreme</u> of the protein that plays an important role during the transport of membrane proteins. A signal as simple as one amino acid in the c-terminal extreme is responsible for the correct transport of the molecule through the whole traject until it reaches the membrane.  

4 0
3 years ago
Pepsin is a digestive enzyme. It is made in an inactive form, pepsinogen, which is activated by the acidity of the stomach to se
Paladinen [302]

Answer: Positive feedback loop

Explanation:

Even before the food reaches the stomach (before ingesting it), the glands of the stomach mucosa begin to release its gastric secretion. The main characteristic of this secretion is acidity, as measured by pH (one of the physiological variables).

This acidity is a result of the presence of hydrochloric acid, which is part of the secretion composition. In addition to it, water, pepsinogen (which will give rise to the enzyme pepsin) and the intrinsic factor. The acid has a function of the protection of the entire system by eliminating microorganisms. It is also responsible for the activation of pepsin (which only occurs with acid pH), the enzyme that digests proteins containing the amino acids leucine or phenylalanine or tryptophan or tyrosine.

The concept of Positive feedback loop states that the body tries to increase the value of a variable (acidity, in the case of pepsinogen) when it is below its optimal value (called a point adjustment) and decreases this value when it is above optimal.

5 0
3 years ago
Which of the following rock types would be more likely to have a course grained texture
Brilliant_brown [7]

Answer:

granite

Explanation:or basalt

please thanks this answer by pressing the heart

8 0
3 years ago
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