Active transport means the molecules move against a concentration gradient from areas of lower concentration to areas of higher concentration.
Answer: After the experiment in step 5
Explanation:
In a scientific investigation, first step is to formulate a question. Second step is to do background research. In third step after background research, a hypothesis is constructed. In order to test hypothesis, an experiment is designed and performed. In the fifth step, data is collected from the experiment and in the last step, conclusions are drawn from the collected data.