Step 1: Isolate the two kinds of DNA.
Step 2: Treat the plasmid and foreign DNA with the same restriction enzyme.
Step 3: Mix the foreign DNA with chopped plasmids.
Step 4: Add DNA ligase.
The rapid release of air/ gas bubbles during cooling
Answer: I am confused maybe add some choices next time please and thank you
Explanation:
No there are no vacuoles in a human but there are in a plant but not humans.