The longest phase of mitosis is prophase. Because the nuclear membrane disappears, Nucleolus disintegrates, and the DNA condensed to form chromosomes (each chromosome is composed of sister chromatids attached around centromere.)
Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
The correct answer is: True