<span>A In spring and summer to coincide with the growing season
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Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
D
Explanation:
All of the other examples are positive
Answer:
Abiotic factors such as latitude and temperature can impact biotic aspects of food web structure like the number of species, the number of links, as well as the proportion of basal or top species. These biotics factors can in turn influence network-structural aspects like connectance, omnivory levels or trophic level. In this way, plants make, or produce, the beginnings of most of the food energy on Earth. This is why plants are called producers. They use some of the food energy to carry out their own functions, and store the rest of the energy in their leaves, stems, roots and other parts.
Explanation:
