Question

An investigator wants to understand whether a newly found membrane protein is involved in membrane transport of a certain particle. Which investigation will help determine whether the new membrane protein is a channel protein involved in membrane transport?
A. Add small nonpolar molecules to the extracellular space and measure the direction of particle movement of the molecules.
B. Measure the rate of extracellular fluid movement into the intracellular space.
C. Add more of the proteins to the plasma membrane and measure the rate of the particle movement
D. Remove ATP from the intracellular space and measure the rate of the particle movement into the intracellular space.

Answer 1

Answer: option D - Remove ATP from the intracellular space and measure the rate of the particle movement into the intracellular space.

Explanation:

In biological membranes, Channel protein like ion channels REQUIRES energy in form of ATP to drive the transport of solutes IN or OUT of the cell.

Usually, ATP is needed to drive transport of solutes against concentration gradient.