Answer: i believe it’s A and C
Explanation: i took biology and remember these lessons
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
see below hope this helps !
Explanation:
Asexual reproduction involves one parent and produces offspring that are genetically identical to each other and to the parent.
Sexual reproduction involves two parents and produces offspring that are genetically unique.
The right answer is C. thermoregulation and hormone transport.
The cardiovascular system has the function of distributing the blood to the organs.
Blood is a red and slightly viscous liquid that circulates in the blood vessels, propelled by the heart, is essential to the maintenance of life. It transports nutrients, oxygen and hormones to the cells of the body, and rids them of their waste.
Blood circulation helps control body temperature and regulates the volume of certain liquids in the tissues. In addition, the blood carries white blood cells, which defend our body against germs.
I believe that the youth movement originated with the baby boomers, the huge generation born after the world war II. By 1970, 58.2 % of the population in America was under the age of 35 years. The economic boom of the 1950s meant more families could afford to send their children to College. College life gave the young people a sense of freedom and independence. It was on college campuses across the nation that youth protest movements began and reached their peak.
