The standard plate count (SPC) method involves diluting 1.0m of bacterial culture into a series of water blanks, and then taking a sample from the water blanks to add to empty petri plates which will be filled with melted agar.
The standard plate count is a method used in microbiology, which is used to gain an insight to estimate the density of bacterial population which is present in a bacterial culture broth. This is done by plating a small concentration of the culture in a petri-dish and then counting the colonies which form in the petri-plate. This method is used mostly in the food industry, to find the density of mesophilic bacteria in food. This method is extremely essential to determine the primary source of the bacterial contaminant.
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The three major islands are Japan, Indonesia and New Zealand.
Malleable because the rest are chemical
I think it D.100% because the air has reached its limit
Answer:
A. Glucose:
As lactose is absent will follow the glucose metabolism, so the lac repressor will hold tightly with the operator which leads to the prevention of transcription as RNA polymerase can not bind to promoter so no lactose metabolism.
B. lactose:
In the case or wild mutant lactose metabolism will occur as lactose is present, allolactose binds to the lac repressor thus the operator and promoter are free so transcription starts. The same result will be in all test except in the case of a mutant repressor.
C. glucose and lactose:
In this case, diauxic growth will occur. This growth takes place when two sugars are present in the media. The preferred sugar will be consumed first That is glucose. Then a lag phase will come which will be followed by the second sugar metabolism that is lactose. the given two cases :
Lactose will be metabolized first, as operator site mutation prevents repressor protein binding and thus will lead to the constitutive synthesis of lac operon in test B.
The same thing will happen, as in this case also, constitutive synthesis of lac operon will happen in test C.
Cultures grown in the presence of both glucose and lactose are metabolizing lactose. In the test (b) and (c) will be responsible due to constitutive synthesis of lac operon will happen.
