Question

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-Iodine

Answer 1

Answer: Gram staining is arranged in the following order;

1) Crystal violet

2) iodine

3) Alcohol-acetone

4) safranin

Explanation:

Applying primary stain to a heat fixed smear of a bacterial culture

Then you add iodine which binds the crystal violet and traps it in the cell. After you decolourise with ethanol finally counter shade with safranin