All categories

3 years ago

The first step of PCR

Biology

1 answer:

Lorico [155]3 years ago

7 0

Answer:

First step:

B. DNA is heated to break the hydrogen bonds that hold the two strands together

Second step:

D. DNA is cooled to allow the primers to bind to the DNA strands Primers

Explanation:

Polymerase chain reaction, or commonly called PCR, is a molecular technique invented by Katy Mullis in the 1980's. It is used to amplify a sequence of DNA template to form several copies.

The process of polymerase chain reaction occurs in three major steps viz;

Step 1: In this step, the DNA template is denatured by heating it to a high temperature. This is done to separate the double stranded molecule into single strands by breaking the hydrogen bonds holding the nucleotides base together.

Step 2: Next, the temperature is reduced or allowed to cook in order for short DNA fragments called PRIMERS to bind to the site on the DNA strands. This step is called ANNEALING.

You might be interested in

The ______ ______ ______ are the only blood cells that function entirely in blood vessels.

oee [108]

The answer is red blood cells

6 0

3 years ago

What methods are you using to test this (or each) hypothesis?

gtnhenbr [62]

Answer: State the hypotheses. Every hypothesis test requires the analyst to state a null hypothesis and an alternative hypothesis.

Formulate an analysis plan. The analysis plan describes how to use sample data to accept or reject the null hypothesis.

Analyze sample data.

Interpret the results.

Explanation:

8 0

3 years ago

Importance of protein in metabolism

Margaret [11]

Answer:

Protein is also used for growth and repair. Amid all these necessary functions, proteins also hold the potential to serve as a metabolic fuel source. Proteins are not stored for later use, so excess proteins must be converted into glucose or triglycerides, and used to supply energy or build energy reserves.

3 0

1 year ago

Which conclusion is best supported by the data above?

Bezzdna [24]

Answer: C. there is more silicon than calcium at the surface of the earth

Explanation:

5 0

3 years ago

Match the reproductive structures based on their function and the system to which they belong.

Rashid [163]

Complete question: Find the complete question in the attached file below.

Answer: From the question, the system to which the structures belong are:

Female reproductive structures include: Egg, ovary, fallopian tube, vagina.

Male reproductive structures include: Urethra, sperm, testis, Vas deferens

Explanation:

The female reproductive system is made up of structures, listed above, which functions together with the purpose of reproducing offsprings. Below is the structure matched with the correct function from the question given:

--> Egg: This functions as the sex cell or gamete

--> ovary: The site where gamete are produced

--> Fallopian tube: This functions to transport the gamete from the site of production in the reproductive system

--> Vagina: connects the internal reproductive fluids to the exterior.

The male reproductive system are also made up of structures that functions together to bring about the fertilization of the female sex cell. Below is the structure matched with the correct function from the question given:

--> Urethra: connects the internal reproductive fluids to the exterior.

--> Sperm:This functions as the sex cell or gamete

--> Testis: The site where gamete are produced

--> Vas deferens: This functions to transport the gamete from the site of production in the reproductive system.

7 0

3 years ago