Answer: The cell wall surrounds the plasma membrane of plant cells and provides tensile strength and protection against mechanical and osmotic stress.
Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Mafia gain the interest and scrutiny of the public through their unique and fearful actions.
Mafia gain the interest and scrutiny of the public by threatening people in the society as well as killing some of them. The main aim of Mafia is to earn money so they perform many actions in order to gain money from the public such as extortion, kidnapping etc.
The person who gives money to Mafia is safe but that person who refuses to pay money to Mafia will gain the punishment of death so we can say that in this way Mafia gain the interest of the public.
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