I believe it is number 3.
Hope this helps!
Answer:
The gene for human insulin is inserted into the gap in the plasmid. this is done through the use of restriction enzymes and ligase to insert and close the plasmid. This plasmid is now genetically modified. The genetically modified plasmid is introduced into a new bacteria or yeast cell. The more the cells divide, the more insulin is produced as bacteria divides into two identical daughter cells like in mitosis.
Explanation:
Established on the data, sickle cell hemoglobin displays altered primary structure and altered quaternary structure; the secondary and tertiary structures may or may not be altered. The sickle cell disease is a cluster of disorders that disturbs hemoglobin, the molecule in red blood cells that transports oxygen to cells all over the body. A person with this disorder have uncharacteristic hemoglobin molecules named hemoglobin S which can interfere with red blood cells into a sickle or crescent shape.
