Answer;
-Cougar
Explanation;
-The amount of biomass decreases from the lowest trophic level to the highest. In all the ecological pyramids, producers (organisms that produce their own food) are found at the lowest trophic level. This trophic level contains the highest amount of energy.
-The organisms in the highest trophic level receives the lowest amount of energy. This is because only 10% of energy moves from one level of the food pyramid to the next. When heat comes from the sun. 90% of it is lost to the environment, 10% is consumed by the plants (primary producer). As a primary consumer ingests the plant, 90% of the original heat from the sun is lost to the environment and the primary consumer receives 10%. The secondary consumer receives 10% and 90% is lost, and so forth.
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Answer:
the diagram explains the process of DNA digestion and DNA ligation, which is usually used in molecular cloning techniques
Explanation:
Molecular cloning can be defined as the process used to synthesize multiple copies of a particular DNA fragment. Molecular cloning requires the insertion of a foreign DNA fragment into an appropriate vector (e.g., a plasmid) through the action of specific enzymes that serve to cut and ligate DNA fragments. DNA digestion and DNA ligation use specific restriction enzymes and DNA ligases, respectively, in order to insert the foreign DNA fragment. For this purpose, restriction enzymes that generate single-stranded overhangs are preferred to create sticky ends which bind by complementary base pairing. Subsequently, a DNA ligase enzyme joins the DNA fragments together in order to create recombinant DNA molecules. DNA Ligation is often achieved by using a specific T4 DNA ligase, while there are many restriction enzymes that generate sticky-ends (e.g., BamHI, EcoRI, BaI228I, etc).
Use warm water, keep the dishes close, and skip the pre-rinse are some ways to save water.
