the diagram explains the process of DNA digestion and DNA ligation, which is usually used in molecular cloning techniques
Explanation:
Molecular cloning can be defined as the process used to synthesize multiple copies of a particular DNA fragment. Molecular cloning requires the insertion of a foreign DNA fragment into an appropriate vector (e.g., a plasmid) through the action of specific enzymes that serve to cut and ligate DNA fragments. DNA digestion and DNA ligation use specific restriction enzymes and DNA ligases, respectively, in order to insert the foreign DNA fragment. For this purpose, restriction enzymes that generate single-stranded overhangs are preferred to create sticky ends which bind by complementary base pairing. Subsequently, a DNA ligase enzyme joins the DNA fragments together in order to create recombinant DNA molecules. DNA Ligation is often achieved by using a specific T4 DNA ligase, while there are many restriction enzymes that generate sticky-ends (e.g., BamHI, EcoRI, BaI228I, etc).
